不同处理方法对刺梨果渣可溶性膳食纤维结构和体外降脂功能的影响Effects of Different Treatments on the Structure and in Vitro Hypolipidemic Function of Soluble Dietary Fiber from Rosa roxburghii Tratt Pomace
朱仁威,谭沙,刘庆庆,施伽,吴小念
摘要(Abstract):
本文以刺梨果渣为原料,分别经普通粉碎(general grinding,GG)、超微粉碎(superfine grinding,SG)、高压均质(high-pressure homogenization,HPH)和超微粉碎协同高压均质(superfine grinding synergistic highpressure homogenization,SG-HPH)处理后,采用酶法提取得到四种刺梨果渣可溶性膳食纤维(Rosa roxburghii Tratt pomace soluble dietary fiber,RRTSDF),分别命名为RRTSDF、S-RRTSDF、H-RRTSDF和SH-RRTSDF,比较不同处理方法得到的四种膳食纤维的得率以及结构和体外降血脂功能。结果表明,与普通粉碎处理对比,其他三种处理方法均能提高RRTSDF得率,其中超微粉碎协同高压均质处理的RRTSDF得率最高(20.48%±0.48%)。四种膳食纤维的中值粒径分别为19.88μm(SH-RRTSDF)<89.49μm(S-RRTSDF)<103.69μm(H-RRTSDF)<107.74μm(RRTSDF)。微观结构表明超微粉碎协同高压均质处理使RRTSDF表面形成多孔隙和皱褶结构。傅里叶变换红外光谱和X射线衍射图谱结果表明,四种处理方法均未改变RRTSDF的官能团和晶型,但是SG、HPH和SH处理均降低了RRTSDF热稳定性,其中SH-RRTSDF热稳定性最差。体外降脂实验结果表明,超微粉碎协同高压均质处理得到的SH-RRTSDF的持油力、模拟胃环境和肠环境的胆固醇吸附能力、牛磺和甘氨胆酸钠的吸附率分别为4.59 g/g、4.03±0.21 mg/g(pH2.0)、10.87±0.20 mg/g(pH7.0)、23.69%±0.06%(牛磺胆酸钠)和33.37%±0.04%(甘氨胆酸钠),显著高于其他三种膳食纤维。综上,超微粉碎协同高压均质处理能有效改善RRTSDF的结构和理化性质,不仅为刺梨果渣膳食纤维提供一种可靠的改性方法,还为刺梨深加工提供理论参考。
关键词(KeyWords): 超微粉碎;高压均质;刺梨;可溶性膳食纤维;结构表征;体外降脂功能
基金项目(Foundation): 贵州省教育厅青年科技人才成长项目(黔教合KY字[2022]063号)
作者(Author): 朱仁威,谭沙,刘庆庆,施伽,吴小念
DOI: 10.13386/j.issn1002-0306.2024060370
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